Embryo Aggregation in Pig Improves Cloning Efficiency and Embryo Quality
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چکیده
منابع مشابه
Oral melatonin supplementation improves oocyte and embryo quality in women undergoing in vitro fertilization-embryo transfer.
The aim of this study was to evaluate the efficacy of oral melatonin supplementation on oocyte and embryo quality in patients in an assisted reproductive technologies program. All patients were treated for at least 2 weeks with melatonin (3 mg/day). To evaluate the cumulative effect of melatonin supplementation, we compared cycle outcomes between the first (no supplementation) and second cycles...
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Porcine embryonic stem cells (pESCs) have become an advantageous experimental tool for developing therapeutic applications and producing transgenic animals. However, despite numerous reports of putative pESC lines, deriving validated pESC lines from embryos produced in vitro remains difficult. Here, we report that embryo aggregation was useful for deriving pESCs from in vitro-produced embryos. ...
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Compared to other mammalian species, pig oocytes and embryos are characterized by high lipid contents stored mainly as lipid droplets in the cytoplasm. This fact has a negative influence on manipulations on oocytes and embryos and, in general, biotechnological procedures are much less advanced in pigs than in cows. This paper discusses current methods for modifying porcine oocytes and embryos u...
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Background: The biochemical characteristics of the follicular fluid (FF) play essential role in oocyte quality, the subsequent fertilization potential and embryo development. The oocyte quality and metabolism largely depends on follicular microenvironment. Present study aimed to investigate the relationship between oocyte quality and FF homocysteine (Hcy) level in women undergoing to IVF treatm...
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Vertebrate ancestors would have increased their egg size to store yolk, and the increase is considered to have altered the cleavage pattern and germ layer formation. Amphibian holoblastic cleavage in which all blastomeres contribute to any one of the three primary germ layers has been widely thought to be a developmental pattern in the stem lineage of vertebrates, and meroblastic cleavage to ha...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0146390